Strycova, who has earned most of her corn as a professional winning doubles titles, beat Konta 7-6, 6-1, which looks pretty bald on paper.
Following the match, however, just as after her recent French Open semi-final defeat by another tricky Czech, Marketa Vondrousova, she slipped back into the "no regrets" mantra.
Just as she did against Sloane Stephens on Saturday, Konta came from a set down to see off her opponent on Centre Court. However, two costly forehand errors from the Brit at 5-5 in the breaker handed Strycova a hard-fought opening set.
Konta, ranked 18th in the world and seeded 19th at Wimbledon, told a reporter he was being "patronizing and disrespectful" after he asked if she "needs to have a look at herself".
Konta attributed Strycova's comeback exclusively to her opponent's good play - and bristled at the notion that it was her own mistakes that were at fault.
Strycova was almost out of the tournament yesterday in the fourth round, though came from a set and 5-2 down against 21st seed Elise Mertens to progress to the last eight, and continued her fine form today against an in-form Konta, hitting just nine unforced errors to 22 winners in a highly impressive performance.
The longest match in Wimbledon history was in 2010, when John Isner beat Nicolas Mahut 70-68 in the fifth set. "I'm really pleased with that". I just wanted to put some tape on it to have extra support.
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Besides the best players from the last 15 years, Kei Nishikori is the only remaining top-10 player in the draw, with Dominic Thiem, Alexander Zverev, Stefanos Tsitsipas, Kevin Anderson, Karen Khachanov and Fabio Fognini all bowing out in the first week.
Now she faces what many regard as the ultimate challenge at Wimbledon, seven-time champion and runner-up here a year ago, Serena Williams.
But before these two women took to court, another player had already confounded the odds.
Something between a sigh and a groan escaped from the crowd as Kvitova pumped her fist and stalked back to her chair leaving Konta to wonder what had just happened and the British crowd to start contemplating what might have been.
"For us it's been an incredible journey to get to this point but it hasn't changed our approach", she said.
He will next play either Sam Querrey or Tennys Sandgren in what will be his 39th appearance in a Grand Slam quarterfinal. Williams, bidding for a 24th Grand Slam title to tie with Australian Margaret Court, will play Riske next after trouncing Carla Suzarez Navarro 6-2, 6-2.
"I am not thinking about sending a message to anyone or the next generation, how they are coming or not".
Konta insisted she had not been as tense as the crowd, saying: "I actually felt quite fine".